Our new study at Current Biology is online! Lindsay Rathbun and Abrar Aljiboury identified that in the zebrafish embryo mitotic centrosomes scale with cell size and are asymmetric in size! We had a great collaboration with the Bembenek Lab at Univ of Michigan along with his student Bai to also examine that this phenomenon is consistent in C. elegans. We had additional great contributions from undergrads to postbacs Nicole Hall and Julie Manikas. Finally we wouldn’t have been able to do any of it if the Amack Lab at SUNY Upstate didn’t introduce us to zebrafish and how awesome they are!
Check out the sweet video of zebrafish embryonic centrosomes:
Microtubules (EMTB-3xGFP) and centrosomes (Centrin-GFP)
The Hehnly Lab’s 1st preprint, led by Lindsay Rathbun with the help of many people including our graduate student Abrar Aljiboury and postbac Julie Manikas, along with Josh Bembenek’s laboratory (University of Michigan with X Bai from the NIH) and Jeff Amack’s laboratory (UPSTATE Medical School, Syracuse NY). We found some crazy large centrosomes in extremely large zebrafish embryo cells that scale with changes in cell size! We didn’t leave out C. elegans either. Check it out here:
Our paper is out! This study was lead by Lindsay Rathbun with many major contributions from past and current lab members and our collaborators Lisa Manning (SU) and Jeffrey Amack (SUNY Upstate). Its a really cool study demonstrating that the final step in cell division, cytokinesis and abscission, is needed for the lumen to form in the zebrafish left-right organizer. This transient tissue goes from a series of mesenchymal like migratory cells that divide and transition into polarized epithelial cells. Our team proposes a model that division assists in this process and the cytokinetic bridge, which can stay around for up to an hour, helps hold the cells in a transient rosette structure before they can initiate lumen formation. Check the paper out here at Nature Communications.
In cyan is a dividing cell interconnected by a cytokinetic bridge that is about to undergo abscission. Once the bridge abscises you can see the KV lumen open up! Magenta is labeling KV cells plasma membrane.
