Michelle, an undergraduate in our lab, gave a great lecture this past week on the pluses and minuses of widefield and laser scanning confocal microscopy in our graduate level course at SU on Microscopy Techniques in Cell Biology. She presented one of my favorite papers by Jason Swedlow that really digs into the advantages of widefield imaging with deconvolution for resolving dim fluorescent structures in live samples. The paper was titled “Measuring tubulin content in Toxoplasma gondii: A comparison of laser-scanning confocal and wide-field fluorescence microscopy” and can be found here.
Check out Erica’s recent publication in Cytoskeleton titled “Regulating a key mitotic regulator, polo-like kinase 1 (PLK1)”. You can find the article here. Here’s her beautiful cover below, which is a Structured Illumination Microscopy Micrograph of PLK1 (Fire Look-up Table) and kinetochores (CREST, white) during different stages of the cell cycle.
Check out the beautiful review titled “The balance between adhesion and contraction during cell division” from Dylan Burnette’s lab, specifically Nilay Taneja, that my graduate student, Lindsay Rathbun, and I were lucky to contribute to. Featured below is a stunning Figure Nilay put together. It’s fantastic and shows the power of Structured Illumination Microscopy.
But, the image we helped them do got featured on the website! Take a read, its pretty cool. http://www.cell.com/molecular-cell/home